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Clear regulatory guidelines and early engagement with agencies are also being pursued to ensure that promising ferroptosis-based CVD therapies can enter clinical trials and, ultimately, patient care

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Sheridan et al., 2011

To confirm GSTP1 knockdown in A2780DPP cells, we isolated individual puromycin-resistant colonies 24 h after colony formation using cloning cylinders, and extracted sufficient RNA to confirm GSTP1 knockdown by qRTPCR analysis (Figure 3B)

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