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Measurements of intracellular ROS After treatment with IL-22 (25 ng/ml) and/or SFN (20 nM), HaCaT cells were harvested and stained with 2 M dihydroethidium (DHE, Beyotime Institute of Biotechnology) for 30 min at 37 C in the dark

Careful, documented calculation is essential for any study that depends on precise dosing in cell culture or animal models, and errors here can meaningfully skew results

de Rothschild, Paris, France, 10 Neurology Department, Hpital Lariboisire, Paris, France, 11 Universit Paris Cit, Paris, France, 12 INSERM, U1144, Paris, France, 13 FHU NeuroVasc, Paris, France, 14 Department of Neurology, Foch Hospital, Versailles Saint-Quentin en Yvelines University, Suresnes, France, 15 Department of Diagnostic and Therapeutic Neuroradiology, Universit de Lorraine, CHRU-Nancy, Nancy, France, 16 Universit de Lorraine, INSERM 1254, IADI, Nancy, France Background and Aims: Time from symptom onset to puncture (TSOP) and time from puncture to reperfusion (TPTR) are associated with worse outcome in ischemic stroke patients treated with endovascular therapy (EVT) in the early window ( 6 h), where EVT may benefit patients with favorable imaging profiles is unknown (late window paradox)

The data also suggests that ibuprofen affects previously unknown pathways such as amino acid, steroid and vitamin metabolism