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*Correspondence: Bonglee Kim, [email protected] These authors have contributed equally to this work Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Other notable techniques include plasma-free amino acid dose-response (Whitehouse et al., 2017), in situ and in vitro methods (Estes et al., 2022), and fecal amino acid excretion (Risnen et al., 2020), all of which are employed to evaluate rumen-protected amino acids

Culture methods are widely used and are generally effective for identifying and quantifying a range of bacteria