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The samples were loaded on the 96-well S-Trap plate (Protifi), placed on top of a deepwell plate, and centrifuged for 2 min at 1,500 x g at RT

Abdominal subcutaneous fat provides easy access for systemic administration

Maintenance of cells and animals Ea.hy926 (E9) cells: E9 cells were purchased from National Centre for Cell Science (NCCS), Pune and maintained in Dulbeccos Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Himedia, A002) (10,000 units of penicillin and 10 mg of streptomycin in 0.9% NaCl) in a humidified atmosphere with 5% CO 2 at 37 C

These receptors stimulate hormones that control blood sugar levels and reduce appetite, triggering weight loss

When L-arginine is metabolized, it produces NO, which promotes vasodilation, improves circulation, enhances oxygen delivery to and oxygenation of tissues, and removes waste