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Methods Protein expression and purification The codon-optimized gene encoding full-length human ABCC2 (Uniprot: Q92887) was synthesized (Sangon Biotech, Shanghai) and cloned into a modified pCAG vector harboring a C-terminal Flag tag (DYKDDDDK) and an 8 His tag using a ClonExpress II One Step Cloning Kit (C113-02, Vazyme Biotech co., Ltd)

In the normal immune environment, latent EBV and the immune system are maintained in a state of equilibrium and when the bodys immune function declines or is exposed to external irritants

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In the same meeting, PCAC voted against the inclusion of the three other substances (ammonium tetrathiomolybdate, enclomiphene citrate, and ferric subsulfate) under review for consideration onto the final 503A Bulks List

Fumel B, Guerquin M-JLG, Staub C, Magistrini M, Gauthier C, Flamant F, Guillou F, Fouchcourt S: Thyroid hormone limits postnatal Sertoli cell proliferation in vivo by activation of its alpha1 isoform receptor (TR1) present in these cells and by regulation of Cdk4/JunD/cmyc mRNA levels in mice