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RT-PCR was performed using LightCycler 480 II Real-time PCR Instrument (Roche, Basel, Switzerland) with QuantiFast SYBR Green qPCR Master Mix (Qiagen, Dsseldorf, Germany)

In addition, it also acts as an acetyl storage, providing energy in carbohydrate and lipid metabolic pathways [48]

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Previous work has shown that Vitamin E supplementation decreased 8-OHdG in muscle of young men but supplementation failed to show a similar effect in elderly men

We attempted to overcome these limitations by focusing on a single pathway, using an information-based gene-selection algorithm (based on location in autism linkage regions and expression patterns), selecting tag-single-nucleotide polymorphisms (SNPs) to achieve very high coverage of genes and flanking regions, and by evaluating high order genegene interactions using a newly developed method adapted for family data