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Decreased arginine levels or excessive NO production suppress T cell proliferation and promote Treg differentiation

Cell proliferation assay The viability of GC-1 spg, TM3 Leydig, and TM4 Sertoli cells was measured using an MTT assay EZ-Cytox kit (Daeil Lab Services Co., Seoul, Korea) according to the manufacturers instructions Each cell was seeded in 24-well plates at a density of 0.5 10 5 cells/well and incubated at 37 C for 12 h

"Selenium: its role as antioxidant in human health." Environ Health Prev Med 13.2 (2008): 102-8

Glutathione has been shown to interfere with tyrosinase activity, reducing the enzymes ability to produce melanin

However, therapeutic application of SIRT1 activators is not without risk