Cell culture and treatment Immortalized human keratinocytes (HaCaT cells), purchased from the China Center for Type Culture Collection, were cultivated in RMPI 1640 medium and complemented with 10% Fetal bovine serum (Gibco, USA), penicillin and streptomycin (NCM, China)
[DOI] [PubMed] [Google Scholar] 143.Rodriguez-Sargent C., Cangiano J., Berros Cabn G., Marrero E., Martnez-Maldonado M
The research was supported in part by the National Institute of General Medical Sciences (R35GM127045 awarded to S.L.S.), the NCIs Cancer Target Discovery and Development (CTD^2) Network (grant number U01CA217848 and U01CA272612 awarded to S.L.S.), and by the NIBR Scholars Program
A tissue-specific approach to the analysis of metabolic changes in Caenorhabditis elegans
More importantly, the experiments of chemical inhibition and cKS were all carried out in RPMI -gln or RPMI Pgln medium containing ~5 times higher asparagine levels (378 M) than plasma (4080 M), suggesting that the extracellular asparagine could not compensate for the endogenous asparagine deficit in Pf caused by GS inhibition