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Tightening the barrier could reduce the negative effects of the inflammation challenge or in the presence of any of the neurodegenerative diseases, controlling the integrity of the BBB could slow the progression of the disease and neurodegeneration

10.1111/j.1471-4159.2005.03053.x J

After removing the membrane and organelle fraction, the pellet was washed 3X with 100L MIB before resuspending the cytoskeletal and nuclear fraction in 125 L Cytoskeletal and Nuclear Isolation Buffer