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Alternatively, for a whole cell lysate, the bacterial pellet was resuspended in the chromatography buffer, lysed by sonication with a Digital Sonifier (Branson Ultrasonics, Danbury, CT) and cleared by centrifugation

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J Biol Chem 300(4):106794

Engel H, Kleine H, Jiang S, Sanchez-Williams G, Andreeff M