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1.6 Biochemical and metabolic profiling The pathogenesis of neurodegenerative disorders generally involves a complex physiopathology that includes age-related changes, abnormal function and accumulation of brain proteins, inflammation, disruption of energy homeostasis, hormonal imbalances, metabolic imbalances, DNA damage, among other processes (Gadhave et al., 2024)

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Why the Glutathione Index Is the Best Indicator of Brain Oxidative Stress The GSH/GSSG ratio reflects the activity of the enzyme glutathione reductase which is responsible for the transformation of GSSG (used, oxidised) to GSH (the reduced or fully loaded form that acts as a radical scavenger)

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The plasmid was mixed 1:3 (w/w) with PEI MAX 40,000 (Polysciences, 24765-1) for 20Lmin at room temperature and then used to transfect Expi293 cells at 37C in 8% CO2 (Thermo A14527)