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The results were expressed as mean SD (standard deviation)

Quantification of gene expression by quantitative PCR Messenger RNA for the pro-oxidant enzymes, the NOX enzymes ( Nox1 , Nox2 , p47 phox , Nox4 ), for the vascular endothelial growth factor-A ( Vegf-a ), for the antioxidant redox enzymes, heme oxygenase 1 ( Ho-1 ), and glutathione peroxidases 1 ( Gpx1 ), for the cytokines, Tnf- , IL-1 , superoxide dismutase type 2 ( Sod2 ), and for the nitric oxide synthase (NOS) isoforms, eNos , iNos , and nNos , was quantified in the whole retinal explants as described before [39]

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Relation between folate status, a common mutation in methylenetetrahydrofolate reductase, and plasma homocysteine concentrations