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Cells were then washed once in PBS-1X, fixed for 10 min in 4% PBS-paraformaldehyde (Electron Microscopy Sciences, 50-980- 495), and washed three times in PBS-1X

The Indian journal of surgery

Despite the advances in therapeutic interventions, numerous inescapable detrimental repercussions, such as the emergence of multi-drug resistance, continue to impede the effectiveness of the treatment regimens (116, 117)