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Given the numerous potential secondary causes, the PIANIST mnemonic (Psychiatric, Iatrogenic/Intoxicant, Anemia, Nutrients, Infection, Sleep, Thyroid/hormonal) can be used to aid in identification (Table 1)

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Mecp2 +/ females, backcrossed more than 14 generations to C57BL/6J males, were bred and male and female pups were assigned to one of the following groups for experiments: male and female wildtype saline-treated (WTS and FWTS, respectively), male and female wildtype ALC-treated (WTA and FWTA, respectively), male null saline-treated (NS), male null ALC-treated (NA), female heterozygous saline-treated (FHETS) and female heterozygous ALC-treated (FHETA)