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Jun 2015;37(3):9794

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Many cells can secrete TC, which combines with free B12 in the cells of the ileum to form holotranscobalamin (holoTC), which enters the portal circulation and is responsible for delivering the vitamin to the cells, as it transports the functional fraction that can be absorbed by the tissues and is rapidly released into them by endocytosis (the process of incorporating molecules into cells) through their specific receptors

Researchers should keep vials sealed until preparation and allow cold materials to equilibrate before opening when condensation risk is present