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After that, the cells were washed with PBS for three times and incubated with 100 L serum-free medium containing 10 L CCK-8 for 1 h

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While there is cogent evidence that SE supplementation may reduce occurrence of these adverse events as well as decrease the risk of AIT complications and prevent PP hypothyroidism, the current lack of clear cut-off levels for Se administration, which implicates the risk of overdose, at present tends to militate against Se supplementation in this setting [68]