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This stable rate of over-volunteering attempts contrasts with the data from a single research site in the UK where the TOPS system was implemented and progressively decreasing over-volunteering rates were observed over time

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We also observed an upregulation of Nos1 (encoding nitric oxide synthase, NOS1) and Ntrk1 (encoding neurotrophic tyrosine kinase receptor 1, TrkA) in this pathway

All experiments were carried out in triplicate, employing ascorbic acid as the reference standard under identical experimental conditions 92,93