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Adhesion assay For cell-substratum adhesion assays, 96-well tissue culture plates were coated with type I collagen (0.5 mg/ml) or fibronectin (0.1 mg/ml) in PBS at RT overnight, rinsed twice with PBS and cells were allowed to adhere to the coated surface for 30 min and 60 min respectively

Hu C-aA: ApoL1, a BH3-only lipid-binding protein, induces autophagic cell death

doi: 10.1093/ajcn/30.7.1009

Read absorbance at 450 nm using a enzyme-labeled instrument (Multiskan FC, Thermo Fisher Scientific, Inc., MA, USA)

It helps relieve the appearance of dryness, itching, and mild irritation associated with eczema-prone skin, while supporting the protective skin barrier and providing long-lasting hydration for up to 24 hours