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Corroborating these findings, immunoblot analysis of whole-kidney lysates demonstrated that UUO-induced upregulation of fibrotic (CCN2, FN1, COL1A1, and COL3A1) and ferroptotic (HMOX1) markers, alongside downregulation of GPX4, was more prominent in WT mice relative to KO littermates (Fig

Denatured proteins are precipitated by cooling and removed via centrifugation, keeping of NAD + , NADH, NADP + , NADPH, GSSG and GSH stable in the supernatant

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