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The translational silencer DJ-1 loses its affinity to RNA when cysteine residues of the DJ-1 protein become oxidized (Blackinton et al., 2009)

Following this, cells were washed with permeabilization buffer (Biolegend) and then stained with intracellular antibody cocktail ( Table 1 ) for 30 mins on ice, wash with Biolegend permeabilization buffer then stained with metal-conjugated streptavidin for 10 mins on ice

10.1254/jphs.SCZ070901 J Pharmacol Sci

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