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We attempted to antagonise this effect on stability by further increasing the net negative charge of scFvs at a lower pH and by decreasing their pI through fusion of the human influenza hemagglutinin (HA) tag (YPYDVPDYA) with low pI (3.53) and a net negative charge (2.2) to the C-terminus of s3Flag-scFv-A36 or s3Flag-scFv-M4 (Fig