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Briefly, pancreatic tissue was digested in Geys balanced salt solution containing 0.75 mg/mL collagenase P (Sigma-Aldrich), 0.03% protease (Roche, Mannheim, Germany), and 0.01% DNase I (Roche)

Through alterations in the circulation and operation of central nervous system neurotransmitters, the gut microbiota can greatly affect the physiology of the hypothalamic-pituitary-thyroid axis

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The authors also wish to acknowledge Ms