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For pre-training data with three experimental groups, one-way analysis of variance (ANOVA) was used to compare effects between CON-CHOW, STZ-CHOW and STZ-KETO

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Antioxidant markers, including superoxide dismutase (SOD), glutathione peroxidase (GPx), and Glutathione (GSH), were assessed using the Superoxide Dismutase Activity Assay Kit (ab65354, Abcam, Shanghai, China), Glutathione Peroxidase Assay Kit (ab102530, Abcam, Shanghai, China), and Glutathione Assay Kit (ab65322, Abcam, Shanghai, China), respectively

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GHK-Cu plays a prominent role in skin regeneration and wound healing, while BPC 157 and TB500 are commonly studied in the context of musculoskeletal and soft tissue recovery