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Acknowledgments This article was supported by the Key Scientific Research Fund of the Hunan Provincial Department of Education (No.: 22A0262), the Scientific Research Project of the Hunan Provincial Health Commission (No.: 202203105682), the Hunan Traditional Chinese Medicine Research Fund (No.: D2022104), the Hunan Provincial Natural Science Foundation of China (No.: 2025JJ90060), the fifth batch of the 2022 National Advanced Training Program for Outstanding Clinical Talents in Traditional Chinese Medicine (Document No

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If the calf was healthy, it was either left to suckle colostrum directly from the dam, with intake confirmed by observation of active suckling behaviour and evidence of udder emptying, or was separated and fed 12 L of frozen colostrum or 300500 g of colostrum replacer (CR) using either a bottle or an oesophageal tube feeder, depending on individual farm protocol