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20 mg vial Concentration: 20 mg 3 mL = 6.667 mg/mL (equivalently 6,667 mcg/mL) Dose in mg: 250 mcg = 0.25 mg Draw volume: 0.25 mg 6.667 mg/mL = 0.0375 mL Syringe units: 0.0375 mL 100 = 3.75 units 4 units on a U-100 syringe On the 20 mg vial, one documented dose is a very small draw about the 4-unit mark

Tryptophan (Trp), an essential amino acid, functions not only as a fundamental substrate for protein biosynthesis but also as a precursor in several critical metabolic pathways and molecules such as serotonin

It depends on both copper and zinc as cofactors, but only copper regulates its activity (Harris, 1992)

It's one of the few peptides encoded in mitochondrial DNA rather than nuclear DNA, which makes it a unique and highly researched tool in metabolic and longevity protocols

Toxicity studies indicate acute and chronic administration of DIBI has no adverse effects on the eyes