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According to the manufacturer's instructions, siRNA was dissolved in 1mL sterile DNAse/RNAse free water (Sigma-Aldrich)

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The upper phase was discarded, the lower phases from the two centrifugations were combined, centrifuged and finally transferred to a new Eppendorf reaction vessel

[DOI] [PMC free article] [PubMed] [Google Scholar] 22.Sikiric P, Seiwerth S, Brcic L, Blagaic AB, Zoricic I, Sever M, Klicek R, Radic B, Keller N, Sipos K, et al

MEF2C, a transcription factor that facilitates learning and memory by negative regulation of synapse numbers and function