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When the dominant bacterial species in the gut is altered, this results in instability of the gut microbiota and an immune reaction within the gut mucosa (82)

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All cell lines were maintained in a humidified atmosphere (containing 5% at 37 C) and in the following media supplemented with 10% fetal bovine serum (FBS): PA-TU- 8902 and Mia PaCa-2 in DMEM, BxPC-3 in RPMI