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Overexpression of GR leads to increases in antioxidant capacity and resistance to photoinhibition [14]

Moreover, Hb polymers were detected in herniated nucleus pulposus using a high-mass detector, marking the formation of ferryl Hb (oxidized Hb), which distinguishes heme iron from ferryl Hb

For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method

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doi: 10.1038/s41568-021-00336-2 81 LeppAMTikkaSHuuhtanenJKaustioMSalmenniemiUKoskenvesaPet al