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Subsequently proteins were transferred onto a nitrocellulose membrane (BioTraceNT, Pall Life Sciences, Dreieich, Germany) by tank blotting (transfer buffer: 20 mM Tris/HCl, pH 8.7, 150 mM glycine and 20% (v/v) methanol)

To further investigate the role of EV-derived VDAC1 protein in modulating the AMPK/mTOR signaling pathway through intercellular transfer, the activity of this pathway was assessed in AGS cells incubated with EVs derived from AGSR cells with or without VDAC1 knockdown

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For Glutathione in Whole Blood

Cancer cells are characterized by their rapid proliferation, primarily fueled by the consumption of glucose as an energy source