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Gel electrophoresis and immunoblotting Whole-cell lysate samples were prepared by lysing cells in RIPA buffer with phosphatase and protease inhibitors (Pierce protease and phosphatase inhibitor tablets) after washing three times with dPBS

Figure 5 4 Discussion The widespread application of semen cryopreservation technology has effectively prolonged sperm lifespan, overcoming temporal and geographical constraints while maximizing the preservation of superior genetic resources from elite breeding males