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Each condition was realized in triplicate and the experiment was repeated four times

We understand a one-size-fits-all approach doesnt always work

The PCs were detected at 254 nm and quantified by comparing them with standard curves prepared with known concentrations of glutathione (GSH), phytochelatin-2 (PC 2 ), phytochelatin-3 (PC 3 ), and phytochelatin-4 (PC 4 )