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Regulation of TORC1 in Response to Amino Acid Starvation via Lysosomal Recruitment of TSC2

L1-Red/ L1-DiS/ L1-CCA input phage (~ 10 11 ) and streptavidin-coated magnetic Dynabeads (50 L/ selection, prewashed with 1 mL PBS) were incubated with blocking buffer (volume made up to 500 L for phage, 1 mL for beads) for 1 h on a rotary mixer at room temperature and 20 RPM

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Importantly, GQD processing reduced the distribution of -syn in the brain (Fig