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The lysate was then allowed to cool to room temperature for 5 min, and a further 4.7 l of 10X glyco-buffer 3 (500 mM sodium acetate pH 6) and 2 l of Endo H enzyme were added and incubated at 37 C overnight

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This nutrient symphony is impossible to replicate with isolated supplements and represents millions of years of evolutionary adaptation to marine food sources