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Samples were spun at low (3220 g , 4 C, 20 min) and high speed (10,000 g , 20 C, 10 min), then incubated with 1.5 ml NiNTA beads (Thermo Fisher Scientific, Waltham, MA) for 1 h at 4 C with rotation

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Gut dysbiosis induced mucus layer erosion, thereby elevating IECs exposure to food antigens and OS, which potentiated Notch signaling activation