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doi: 10.1056/NEJMra0808284 4 BarrettJCClaytonDGConcannonPAkolkarBCooperJDErlichHAet al

Current research suggests: 5-Amino-1MQ does not primarily work by reducing appetite

The soluble fraction was clarified by centrifugation (75,000 g , 20 min, 4 C) and incubated with 50% Ni-Sepharose suspension (GE Healthcare, Chalfont St Giles, UK) for 2 h at 4 C with gentle rotation prior to affinity purification