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Mycobacterium tuberculosis infects dendritic cells with high frequency and impairs their function in vivo

Cells were resuspended in RPMI-1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin, seeded into six-well plates, and treated with the indicated compounds for 3 days

For macrophage polarization experiments, cells were then treated with either human recombinant IL-4 (10 ng/mL, Cedarlane Labs, Burlington, ON) or IFN- (10 ng/mL, eBioscience, San Diego, CA) for 48 h

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On the other hand, DTP cells display a preference for utilizing mitochondrial respiration and the electron transport chain to acquire energy