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For the live imaging of reverse dimerization, HeLa cells were transfected as above but seeded on -Slide-4-wells coverslips (Ibidi)

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However, unbinding of the product was calculated to be ~7 kcal mol -1 more favorable than the reactant, ensuring catalytic turnover

the antibody or antigen binding portion thereof binds an antigen (e.g., ALPP and/or ALPPL2) or a cell expressing the antigen with an affinity (K D ) of 5 nM or better (i.e., or less) (e.g., 40 nM or 30 nM or 20 nM or 10 nM or less), as measured using a surface plasmon resonance assay or a cell binding assay

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