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doi: 10.1086/320158

10.1016/j.jchf.2015.08.007 145

Behavioral assessments were performed using the elevated plus maze, open field, lightdark box, and hole board tests

The resulting pellet containing restriction enzyme-digested DNA fragments was incubated with 150 M biotin-14-dATP, dCTP, dGTP, and dTTP, and 20 units of Klenow fragment (NEB) at 37 C for 45 min, followed by proximity ligation using 2500 units of T4 DNA ligase (NEB) at room temperature for 4 h with gentle rotation