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2.3 Randomization Participants were randomized (1:1) using a pre-specified block randomization sequence (BABABAABAABBABBA), ensuring balanced allocation across treatment sequences

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ssDNA pool from each round of selection had been previously amplified by PCR using the aforementioned primers and purified as previously described

Vitamin B6 deficiency modulates cysteine synthesis, and it has been observed that supplementation with vit B6 was found to be beneficial because the enzymes CSE and CBS rely on pyridoxal 5-phosphate as a cofactor affects the flow through the reverse TSP

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