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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

5L), underscoring the essential role of SLC25A39 in maintaining mitochondrial integrity and function

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Age Myelination is tightly regulated over the lifespan, with onset in humans in the third trimester and peaking in the first decade, and continuing on in adulthood to support learning and memory processes 86

Any shift toward brown or green means the product has degraded and should be replaced