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The next day, cells were washed and incubated for 90 min at room temperature with Alexa Fluor-conjugated secondary antibodies (anti-mouse Alexa Fluor 555, Invitrogen

After collagen polymerization (1 hour at 5% CO 2 and 37C), each well was filled with 73 l of culture media and left overnight in the incubator for cell acclimatization

Mobile genetic elements, plasmids, and integrons are strongly related to resistance to trimethoprim and sulfamethoxazole (Stephens et al., 2020

Further optimization and validation on larger patient cohorts are necessary to establish its practical utility in the field

Phase Low-moderate Daily dose 20-50 mcg/day Site Trained muscle, post-workout Notes Often used in research interest in localized hypertrophy signaling