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Description

Partial crushes can lead to extensive variability and reduced reliability compared to total crushes

01 Wash and prepare Wash hands thoroughly

Western blotting analysis Tissues or cells were resuspended in lysis buffer on ice for 5 minutes, and the supernatants were collected after centrifugation at 13,000 g for 15 minutes

Notably, the in vivo biodistribution tracking demonstrated pronounced co-localization of DiO-labeled MBC with CA3-positive NP in rat caudal disks (Supplementary Fig