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On the day of the assay, the culture medium was changed for unbuffered DMEM (pH 7.4) supplemented with 2.5 mM glucose and the cells were equilibrated for 10 min at 37 C in a CO 2 -free incubator

Fatty acid desaturase 2 (FADS2) gene encodes a rate limiting enzyme in the synthesis of long chain poly unsaturated fatty acids from essential fatty acids, and FADS2 gene rs174583 single nucleotide polymorphism (SNP) has been reported to be a risk factor in cardiovascular events by affecting hyperglyceridemia, hyperglycemia and hyperinsulinemia

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Back (away from spine): The shoulder blades offer a thick canvas

L.KahlJ.SinghR.Coenen-SchimkeJ.RaghavakaimalS.et al (2005)