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However, NO reaction with superoxide anion can lead to peroxynitrite production [60], which is typically limited by relatively high concentrations of superoxide dismutase [61]

Primary injury is caused by the immediate mass effect of the hematoma, leading to compression, ischemia, and mechanical destruction of surrounding brain tissue 2

Low-fat areas such as the inner thigh, the lower abdomen in leaner individuals, and sites near bony prominences should be avoided where possible as they offer less tissue cushioning and produce more pronounced discomfort

For injectable research use, consistency matters more than exact timing