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IDD can lead to many related and often progressive medical conditions as a result of spinal instability, disc herniation, and nerve impingement such as spondylosis, which refers to degenerative and osteoarthritic changes to the discs and vertebral bones, and sciatica, where pressure put on the sciatic nerve root by a herniated disc or bone spur can cause discomfort, pain, and limb weakness (Mulleman et al., 2006

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At that time, MS technology was also utilized by industrial chemists, who knew the identities and structures of most of the molecules in their mixtures, and used MS for measuring the abundances of small hydrocarbons in process streams (to ensure the correct ratios of components for high-quality fuels and synthetic rubber production), improving quality control, and process monitoring (Griffiths, 2008