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In hepatocytes, carboxyl terminus of HSC70-interacting protein (CHIP) interacts with NIK and TRAF3 and appears to act coordinately with TRAF3 to promote NIK degradation [44]

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BT474 cells and PC3- hTERT cells were cultured in DMEM F-12 medium (Gibco, Invitrogen) with 10% FBS (Gibco, Invitrogen) and 1% PenicillinStreptomycin (Gibco)

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Elevated levels of estrogen might enhance the initial healing phase by promoting cellular activities that regenerate ligament tissues, but they might also alter collagen cross-linking, potentially affecting the long-term strength of the repaired ACL