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DAPI was used for staining of nuclei (Life Technologies)

Enhancing the efficiency and specificity of a genetic vaccine nucleic acid uptake by a given cell type by coating the nucleic acid with an evolved protein that binds to the genetic vaccine nucleic acid, and is also capable of binding to the target cell In one embodiment, the invention provides methods of enhancing the efficiency and specificity of a genetic vaccine nucleic acid uptake by a given cell type by coating the nucleic acid with an evolved protein that binds to the genetic vaccine nucleic acid, and is also capable of binding to the target cell

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Its relevance to neuropathy is most direct in the metabolic neuropathies, particularly diabetic peripheral neuropathy, where impaired glucose metabolism, insulin resistance, and mitochondrial dysfunction all contribute to nerve damage

However, the pronounced irregularity of the spherical vesicles and tubules induced by the SNX1-SNX2a heterodimer hindered the application of iterative helical real-space reconstruction, making it impractical to obtain high-resolution structural information for the complex