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Therefore, to enhance mitochondrial complex I activity, NAD + levels were boosted in fly models of PD with NAM, leading to reduced oxidative stress, including lower ROS levels and DNA damage, as well as improved climbing ability 281

Treatment with 5-amino-1mq upregulates proliferationrelated genes (Ki67, PCNA), promotes cell cycle progression, and improves cell fusion efficiency to form more multinucleated myotubes, achieving functional levels similar to those of young mice.Additionally, 5-amino-1mq inhibits muscle stem cell apoptosis, reduces oxidative stress damage, and maintains stemness