The complex mechanism of glutamate dehydrogenase in insulin secretion
Competing interests The authors declare no conflict of interest
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Immunocytochemistry Following treatment and/or IVM, oocytes were washed in phosphate buffered saline (PBS) containing 3 mg/ml polyvinylpyrrolidone (PBS/PVP) before being fixed in 2% paraformaldehyde (w/v) diluted in PBS/0.5% Triton-X (v/v) for 30 min and prepared for immunostaining
