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After equilibration, the proteins were separated in the second dimension using 12.5% polyacrylamide gels using an MiniVE vertical electrophoresis system (GE Healthcare Life Sciences, UK)

Processing time: forty-eight to seventy-two hours

In 2005, Bapat et al (61) made a substantial breakthrough by isolating and culturing suspended cell spheres with CSC characteristics from ascites obtained from patients with advanced OC

Following the identification of oxidative stress-related genes (HMOX1, DNAJB1, GADD45B), higher expression levels were observed in HepG2 ATP7B-KO cells compared to HepG2 cells

Within the antioxidant defense system, the fat-soluble vitamin E directly neutralizes lipid radicals within membrane phospholipids, effectively halting the chain reaction of lipid peroxidation